Uyilo loBugcisa oluPhezulu lweProteinase K CAS No. 39450-01-6 ngexabiso elihle
Sihlala sisebenza njengabantu ababonakalayo ukuqinisekisa ukuba sikunika eyona nto ilungileyo kunye nexabiso elithengiswayo elihle kakhulu loMgangatho oPhezulu woYilo loBugcisaIproteni K Inombolo ye-CAS 39450-01-6ngexabiso elingcono, Ukuze ufumane ulwazi olongezelelweyo kunye neenkcukacha, nceda uthethe nathi ngokukhawuleza okukhulu!
Sihlala sisebenza njengabantu ababonakalayo ukuqinisekisa ukuba sikunika eyona nto ilungileyo kunye nexabiso eliphantsi lokuthengisa.I-albhamu ye-China Tritirachium Limber kunye ne-Molecular Biology, Ulawulo oluqinileyo lomgangatho luyaqhutywa kwikhonkco ngalinye lenkqubo yonke yemveliso. Sinethemba elikhulu lokuseka intsebenziswano enobuhlobo neyingenelo phakathi kwenu. Ngokusekelwe kwizisombululo ezikumgangatho ophezulu kunye nenkonzo egqibeleleyo yokuthengisa ngaphambi/emva kokuthengisa, yingcamango yethu, abanye abathengi bebesebenzisana nathi kangangeminyaka engaphezu kwemi-5.
| Ulwelo lweNombolo 1 | |
| Umsebenzi othile weproteni>800U/mL, uxinzelelo yi-20mg/mL, akukho ntsalela yeNickase | |
| umgubo wenombolo 2 | |
| Inkangeleko | Umgubo omhlophe ocoliweyo |
| Ubumsulwa be-electrophoretic | ≥95% |
| Umsebenzi we-enzyme | ≥30U/mg |
| Intsalela ye-asidi ye-nucleic | Ayibonakali |
| Intsalela yeDeoxyribonuclease | Ayibonakali |
| Intsalela yeRibonuclease | Ayibonakali |
| Inombolo yenqwelo | G1205-10ML |
| Indawo yokugcina izinto | Xa igcinwe kwi--20 °C, imveliso igcina umsebenzi wayo ubuncinane unyaka omnye, ithuthwa ngeengxowa zomkhenkce. |
Iproteni Kyiprotease ye-serine ekwintsapho ye-subtilisin enomsebenzi we-enzyme osebenzayo kunye nokucaciswa kwe-substrate ebanzi, ekhetha ukubola iibhondi ze-ester kunye neepeptides ezikufutshane ne-C-terminus yee-amino acids ze-hydrophobic, ii-amino acids eziqulethe i-sulfur, kunye neebhondi ze-aromatic amino acids, ezihlala zisetyenziselwa ukonakalisa iiproteni ukuvelisa ii-peptides ezimfutshane.Iproteni Kinophawu lwe-catalytic triad eqhelekileyo i-Asp39-His69-Ser224 ekhethekileyo kwi-serine proteases kwaye ineendawo ezimbini zokubopha ze-Ca2+ ezijikeleze iziko elisebenzayo ukwandisa uzinzo lwayo kunye nokugcina umsebenzi ophezulu we-enzyme phantsi kweemeko ezahlukeneyo.
1. Isixhobo sokuchonga izifo zemfuza.
2. Izixhobo zokukhupha i-RNA kunye ne-DNA.
3. Khupha izinto ezingezizo iiproteni kwizicubu uze wonakalise ukungcola kweeproteni (njengokulungiselela ii-DNA vaccines kunye ne-heparin).
4. Ukulungiswa kweChromosomal DNA yePulse Electrophoresis.
5. Ukucinywa kweNtshona.
6. Uphuhliso kunye nokuveliswa kwee-enzymes ze-glycated albumin reagents kwicandelo lokuxilongwa kwe-in vitro.
1. 30mg/ibhotile 1g/ibhotile okanye 100g/ibhotile
2. 1kgs/ibhegi yefoyile
I-Proteinase K yi-protease ye-serine ezinzileyo eneenkcukacha ezibanzi ze-substrate. Yonakalisa iiproteni ezininzi kwimeko yayo nokuba kukho izicoci. Ayisebenziswa zii-ion zesinyithi, ii-agents ze-chelating (umzekelo, i-EDTA), ii-reagents ze-sulfhydryl, okanye zii-tripsin okanye ii-chymotrypsin inhibitors. Izinzile kuluhlu olubanzi lwe-pH (4–12.5), kunye nomsebenzi olungileyo kwi-pH 6.5–9.5. Umsebenzi unokukhuthazwa ngokongezwa kwee-agents ezinciphisa ubume (SDS kunye ne-Urea). Ukuncipha ngokukhawuleza kwe-enzyme kwenzeka kubushushu obungaphezulu kwama-70°C. Ukuguqulwa kwe-enzyme kwenzeka ngakumbi kwi-alkaline pH. Nangona kunjalo, i-Proteinase K ayisebenzi ngokupheleleyo yi-autolysis. Ezinye iziqwenga ze-enzyme ziyaqhubeka nokugcina umsebenzi wazo opheleleyo we-proteolytic, nangona emva kokuguqulwa okukhulu kwe-autolysis. I-Proteinase K isetyenziswa rhoqo kwizicelo ze-molecular biology ukugaya iiproteni ezingafunekiyo, ezifana nee-nucleases kumalungiselelo e-DNA okanye e-RNA avela kwii-microorganisms, iiseli ezikhuliswe, kunye nezityalo. Le enzyme idla ngokusetyenziswa kwi-50–200 ug/ml kumalungiselelo e-nucleic acid kwi-pH 7.5–8.0 kunye ne-37~55oC. Amaxesha okufukama ahluka ukusuka kwimizuzu engama-30 ukuya kwiiyure ezili-18.
I-serine protease ezinzileyo nesabela kakhulu; isetyenziselwa ukwahlulwa kweproteni kunye ne-nucleic acid. I-Proteinase K isetyenziswa ekucoceni i-RNA kunye ne-DNA kwizicubu okanye kwimigca yeseli.
Sihlala sisebenza njengabantu ababonakalayo ukuqinisekisa ukuba sikunika eyona nto ilungileyo kunye nexabiso eliphantsi lokuthengisa leProteinase K yoYilo loBugcisa ePhakamileyo Nombolo yeCAS 39450-01-6 enexabiso eliphantsi, Ukuze ufumane ulwazi olongezelelweyo kunye neenkcukacha, nceda uthethe nathi ngokukhawuleza okukhulu!
Uyilo loBugcisaI-albhamu ye-China Tritirachium Limber kunye ne-Molecular Biology, Ulawulo oluqinileyo lomgangatho luyaqhutywa kwikhonkco ngalinye lenkqubo yonke yemveliso. Sinethemba elikhulu lokuseka intsebenziswano enobuhlobo neyingenelo phakathi kwenu. Ngokusekelwe kwizisombululo ezikumgangatho ophezulu kunye nenkonzo egqibeleleyo yokuthengisa ngaphambi/emva kokuthengisa, yingcamango yethu, abanye abathengi bebesebenzisana nathi kangangeminyaka engaphezu kwemi-5.
