Uyilo loBuchule oluPhezulu loMgangatho weProteinase K CAS No. 39450-01-6 ngexabiso elihle
Sihlala sisenza ukuba singabasebenzi ababambekayo siqinisekisa ukuba siya kukunika elona xabiso libalaseleyo kunye nelona xabiso lilungileyo lokuthengisa kuYilo oluPhezulu oluPhezulu.Iprotheni K Inombolo ye-CAS 39450-01-6ngexabiso Elihle, Ngolwazi olongezelelweyo kunye neenyani, nceda uthethe nathi ngokukhawuleza!
Sihlala senza ukuba singabasebenzi ababambekayo siqinisekisa ukuba siya kukunika elona ncedo lubalaseleyo kunye nelona xabiso lilungileyo lokuthengisaI-albhamu yaseTshayina ye-Ttirachium Limber kunye ne-Biology yeMolekyuli, Ulawulo olungqongqo lwekhwalithi lwenziwa kwikhonkco nganye yenkqubo yonke yemveliso.Sithemba ngokunyanisekileyo ukuseka intsebenziswano enobuhlobo kunye neyokuncedana kunye nawe. Ngokusekwe kwizisombululo ezikumgangatho ophezulu kunye nenkonzo egqibeleleyo yokuthengisa / emva kokuthengisa ngumbono wethu, abanye abathengi baye basebenzisane nathi ngaphezulu kweminyaka emi-5.
| No.1 ulwelo | |
| Umsebenzi okhethekileyo weprotheyini> 800U/mL, i-concentration yi-20mg/mL, akukho ntsalela ye-Nickase | |
| No.2 umgubo | |
| Imbonakalo | White lyophilized powder |
| Ukucoceka kwe-Electrophoretic | ≥95% |
| Umsebenzi we-enzyme | ≥30U/mg |
| Intsalela ye-Nucleic acid | Ayibonakali |
| Deoxyribonuclease intsalela | Ayibonakali |
| Intsalela yeRibonuclease | Ayibonakali |
| Inombolo yenqwelo. | I-G1205-10ML |
| Ugcino | Xa igcinwe kwi--20 °C, imveliso igcina umsebenzi ubuncinane iminyaka eyi-1, ukuthuthwa kwengxowa yomkhenkce. |
Iprotheni Kyi-serine protease yosapho lwe-subtilisin enomsebenzi osebenzayo we-enzymatic kunye nokucaciswa kwe-substrate ebanzi, ekhethe ukubola iibhondi ze-ester kunye neepeptides ezikufutshane ne-C-terminus ye-hydrophobic amino acids, i-amino acids equlethe isulfure, kunye neebhondi ze-amino acids ezinevumba elimnandi. edla ngokusetyenziswa ukuthoba iiproteni ukuvelisa iipeptides ezimfutshane. Iproteinase K inophawu lwe-catalytic triad eyi-Asp39-His69-Ser224 ekhethekileyo kwi-serine proteases kwaye ineendawo ezimbini ezibophelelayo ze-Ca2+ ezijikeleze iziko elisebenzayo ukwandisa uzinzo nokugcina umsebenzi ophezulu we-enzymatic phantsi koluhlu olubanzi lweemeko.
1. Ikhithi yokuxilongwa kwemfuzo.
2. I-RNA kunye ne-DNA extraction kits.
3. Khipha amacandelo angenayo iprotheni kwizicubu kunye nokunciphisa iiprotheyini ezingcolileyo (ezifana nokulungiselela izitofu ze-DNA kunye ne-heparin).
4. Ukulungiswa kweChromosomal DNA yePulse Electrophoresis.
5. Ukucinywa kwaseNtshona.
6. Uphuhliso kunye nokuveliswa kwe-enzymatic glycated albumin reagents kwintsimi ye-in vitro diagnostics.
1. 30mg / ibhotile 1g / ibhotile okanye 100g / ibhotile
2. 1kgs/ibhegi yefoyile
I-Proteinase K yi-serine protease ezinzileyo ene-substrate ebanzi. Ithoba iiproteni ezininzi kwilizwe lasekuhlaleni naxa kukho izinto zokucoca. Ayinakwenziwa i-ion zetsimbi, i-chelating agents (umzekelo, i-EDTA), i-sulfhydryl reagents, okanye i-tripsin okanye i-chymotrypsin inhibitors. Izinzile kuluhlu olubanzi lwe-pH (4-12.5), kunye nomsebenzi ofanelekileyo kwi-pH 6.5-9.5. Umsebenzi unokukhuthazwa ngokongezwa kwee-arhente ze-denaturing (SDS kunye ne-Urea). Ukukhutshwa ngokukhawuleza kwe-enzyme kwenzeka kwiqondo lokushisa elingaphezu kwama-70 ° C. I-autolysis ye-enzyme yenzeka ngokunyukayo kwi-alkaline pH. Nangona kunjalo, iProteinase K ayenziwanga isebenze ngokupheleleyo ngokuzihlaziya. Ezinye iziqwenga ze-enzyme ziyaqhubeka nokugcina umsebenzi wazo opheleleyo weproteolytic, nasemva kokwenziwa kwe-autolysis. Iproteinase K isetyenziswa rhoqo kwizicelo zebhayoloji yemolekyuli ukugaya iiproteni ezingafunekiyo, ezinjengee-nucleases kwi-DNA okanye amalungiselelo e-RNA asuka kwi-microorganisms, iiseli ezikhuliswe kunye nezityalo. I-enzyme isetyenziswa ngokuqhelekileyo kwi-50-200 ug / ml kumalungiselelo e-nucleic acid kwi-pH 7.5-8.0 kunye ne-37 ~ 55oC. Amaxesha okufukamela ahluka ukusuka kwimizuzu engama-30 ukuya kwiiyure ezili-18.
I-serine protease ezinzileyo kwaye esebenza kakhulu; isetyenziselwa iprotheni kunye ne-nucleic acid isolation.I-Proteinase K isetyenziselwa ukuhlanjululwa kwe-RNA kunye ne-DNA kwizicubu okanye imigca yeeseli.
Sidla ngokwenza ukuba ngabasebenzi ababambekayo siqinisekisa ukuba siya kukunika eyona nzuzo ibalaseleyo kunye nelona xabiso lilungileyo lokuthengisa loYilo oluPhezulu loMgangatho weProteinase K CAS No. kuthi ngokukhawuleza kangangoko kunokwenzeka!
Uyilo lobuNgcaliI-albhamu yaseTshayina ye-Ttirachium Limber kunye ne-Biology yeMolekyuli, Ulawulo olungqongqo lwekhwalithi lwenziwa kwikhonkco nganye yenkqubo yonke yemveliso.Sithemba ngokunyanisekileyo ukuseka intsebenziswano enobuhlobo kunye neyokuncedana kunye nawe. Ngokusekwe kwizisombululo ezikumgangatho ophezulu kunye nenkonzo egqibeleleyo yokuthengisa / emva kokuthengisa ngumbono wethu, abanye abathengi baye basebenzisane nathi ngaphezulu kweminyaka emi-5.
