mveliso ixabiso eliphantsi Alkaline ProteinaseK CAS 39450-01-6
Sizibophelele ekunikezeni ireyithi yokukhuphisana, iimveliso ezikhethekileyo kunye nezisombululo ezikumgangatho ophezulu, ngokunjalo nokuhanjiswa okukhawulezayo kwexabiso eliphantsi lealkaline.ProteinaseK CAS 39450-01-6, Besifuna ngokunyanisekileyo phambili ukusebenzisana nabathengi kuyo yonke indawo kwihlabathi liphela. Sikholelwa ukuba siya kukwanelisa. Sikwamkele ngokufudumeleyo abathengi ukuba baye kumbutho wethu kwaye bathenge iimveliso zethu.
Sizibophelele ekunikezeni izinga lokhuphiswano, iimveliso ezikhethekileyo kunye nezisombululo ezikumgangatho ophezulu, ngokunjalo nokuhanjiswa ngokukhawulezaI-China Alkaline ProteinaseK kunye ne-39450-01-6, Ngenxa yomgangatho olungileyo kunye namaxabiso afanelekileyo, iimveliso zethu ziye zathunyelwa kumazwe angaphezu kwe-10 kunye nemimandla. Sijonge phambili ekusebenzisaneni nabo bonke abathengi abasuka ekhaya nakwamanye amazwe. Ngaphezu koko, ukwaneliseka kwabathengi kukufuna kwethu ngonaphakade.
| No.1 ulwelo | |
| Umsebenzi okhethekileyo weprotheyini> 800U/mL, i-concentration yi-20mg/mL, akukho ntsalela ye-Nickase | |
| No.2 umgubo | |
| Imbonakalo | White lyophilized powder |
| Ukucoceka kwe-Electrophoretic | ≥95% |
| Umsebenzi we-enzyme | ≥30U/mg |
| Intsalela ye-Nucleic acid | Ayibonakali |
| Deoxyribonuclease intsalela | Ayibonakali |
| Intsalela yeRibonuclease | Ayibonakali |
| Inombolo yenqwelo. | I-G1205-10ML |
| Ugcino | Xa igcinwe kwi--20 °C, imveliso igcina umsebenzi ubuncinane iminyaka eyi-1, ukuthuthwa kwengxowa yomkhenkce. |
Iprotheni Kyi-serine protease yosapho lwe-subtilisin enomsebenzi osebenzayo we-enzymatic kunye nokucaciswa kwe-substrate ebanzi, ekhethe ukubola iibhondi ze-ester kunye neepeptides ezikufutshane ne-C-terminus ye-hydrophobic amino acids, i-amino acids equlethe isulfure, kunye neebhondi ze-amino acids ezinevumba elimnandi. edla ngokusetyenziswa ukuthoba iiproteni ukuvelisa iipeptides ezimfutshane. Iproteinase K inophawu lwe-catalytic triad eyi-Asp39-His69-Ser224 ekhethekileyo kwi-serine proteases kwaye ineendawo ezimbini ezibophelelayo ze-Ca2+ ezijikeleze iziko elisebenzayo ukwandisa uzinzo nokugcina umsebenzi ophezulu we-enzymatic phantsi koluhlu olubanzi lweemeko.
1. Ikhithi yokuxilongwa kwemfuzo.
2. I-RNA kunye ne-DNA extraction kits.
3. Khipha amacandelo angenayo iprotheni kwizicubu kunye nokunciphisa iiprotheyini ezingcolileyo (ezifana nokulungiselela izitofu ze-DNA kunye ne-heparin).
4. Ukulungiswa kweChromosomal DNA yePulse Electrophoresis.
5. Ukucinywa kwaseNtshona.
6. Uphuhliso kunye nokuveliswa kwe-enzymatic glycated albumin reagents kwintsimi ye-in vitro diagnostics.
1. 30mg / ibhotile 1g / ibhotile okanye 100g / ibhotile
2. 1kgs/ibhegi yefoyile
I-Proteinase K yi-serine protease ezinzileyo ene-substrate ebanzi. Ithoba iiproteni ezininzi kwilizwe lasekuhlaleni naxa kukho izinto zokucoca. Ayinakwenziwa i-ion zetsimbi, i-chelating agents (umzekelo, i-EDTA), i-sulfhydryl reagents, okanye i-tripsin okanye i-chymotrypsin inhibitors. Izinzile kuluhlu olubanzi lwe-pH (4-12.5), kunye nomsebenzi ofanelekileyo kwi-pH 6.5-9.5. Umsebenzi unokukhuthazwa ngokongezwa kwee-arhente ze-denaturing (SDS kunye ne-Urea). Ukukhutshwa ngokukhawuleza kwe-enzyme kwenzeka kwiqondo lokushisa elingaphezu kwama-70 ° C. I-autolysis ye-enzyme yenzeka ngokunyukayo kwi-alkaline pH. Nangona kunjalo, iProteinase K ayenziwanga isebenze ngokupheleleyo ngokuzihlaziya. Ezinye iziqwenga ze-enzyme ziyaqhubeka nokugcina umsebenzi wazo opheleleyo weproteolytic, nasemva kokwenziwa kwe-autolysis. Iproteinase K isetyenziswa rhoqo kwizicelo zebhayoloji yemolekyuli ukugaya iiproteni ezingafunekiyo, ezinjengee-nucleases kwi-DNA okanye amalungiselelo e-RNA asuka kwi-microorganisms, iiseli ezikhuliswe kunye nezityalo. I-enzyme isetyenziswa ngokuqhelekileyo kwi-50-200 ug / ml kumalungiselelo e-nucleic acid kwi-pH 7.5-8.0 kunye ne-37 ~ 55oC. Amaxesha okufukamela ahluka ukusuka kwimizuzu engama-30 ukuya kwiiyure ezili-18.
I-serine protease ezinzileyo kwaye esebenza kakhulu; isetyenziselwa iprotheni kunye ne-nucleic acid isolation.I-Proteinase K isetyenziselwa ukuhlanjululwa kwe-RNA kunye ne-DNA kwizicubu okanye imigca yeeseli.
Sizibophelele ekunikezeni izinga lokhuphiswano, iimveliso ezikhethekileyo kunye nezisombululo ezikumgangatho ophezulu, ngokunjalo nokuhanjiswa okukhawulezayo kwexabiso eliphantsi lomzi-mveliso weAlkaline ProteinaseK.CAS39450-01-6, Besifuna ngokunyanisekileyo phambili ukusebenzisana nabathengi kuyo yonke indawo kwihlabathi liphela. Sikholelwa ukuba siya kukwanelisa. Sikwamkele ngokufudumeleyo abathengi ukuba baye kumbutho wethu kwaye bathenge iimveliso zethu.
ixabiso eliphantsi lomzi-mvelisoI-China Alkaline ProteinaseK kunye ne-39450-01-6, Ngenxa yomgangatho olungileyo kunye namaxabiso afanelekileyo, iimveliso zethu ziye zathunyelwa kumazwe angaphezu kwe-10 kunye nemimandla. Sijonge phambili ekusebenzisaneni nabo bonke abathengi abasuka ekhaya nakwamanye amazwe. Ngaphezu koko, ukwaneliseka kwabathengi kukufuna kwethu ngonaphakade.
