ixabiso eliphantsi lomzi-mveliso iAlkaline ProteinaseK CAS 39450-01-6
Sizimisele ukubonelela ngexabiso eliphantsi, iimveliso kunye nezisombululo ezikumgangatho ophezulu, kunye nokuhanjiswa ngokukhawuleza kweAlkaline kwifektri ngexabiso eliphantsi.I-ProteinaseK CAS 39450-01-6Besisoloko sifuna ukusebenzisana nabathengi kwihlabathi liphela. Sikholelwa ukuba siza kukwanelisa. Sikwabamkela ngobubele abathengi ukuba baye kumbutho wethu baze bathenge iimveliso zethu.
Sizimisele ukubonelela ngexabiso eliphantsi, iimveliso kunye nezisombululo ezikumgangatho ophezulu, kunye nokuhanjiswa ngokukhawulezaI-China Alkaline ProteinaseK kunye ne-39450-01-6Ngenxa yomgangatho olungileyo kunye namaxabiso afanelekileyo, iimpahla zethu zithunyelwe kumazwe angaphezu kwe-10 nakwiindawo. Silangazelela ukusebenzisana nabo bonke abathengi basekhaya nakwamanye amazwe. Ngaphezu koko, ukwaneliseka kwabathengi yinto esiyifunayo ngonaphakade.
| Ulwelo lweNombolo 1 | |
| Umsebenzi othile weproteni>800U/mL, uxinzelelo yi-20mg/mL, akukho ntsalela yeNickase | |
| umgubo wenombolo 2 | |
| Inkangeleko | Umgubo omhlophe ocoliweyo |
| Ubumsulwa be-electrophoretic | ≥95% |
| Umsebenzi we-enzyme | ≥30U/mg |
| Intsalela ye-asidi ye-nucleic | Ayibonakali |
| Intsalela yeDeoxyribonuclease | Ayibonakali |
| Intsalela yeRibonuclease | Ayibonakali |
| Inombolo yenqwelo | G1205-10ML |
| Indawo yokugcina izinto | Xa igcinwe kwi--20 °C, imveliso igcina umsebenzi wayo ubuncinane unyaka omnye, ithuthwa ngeengxowa zomkhenkce. |
Iproteni Kyiprotease ye-serine ekwintsapho ye-subtilisin enomsebenzi we-enzymatic osebenzayo kunye nokucaciswa kwe-substrate ebanzi, ekhetha ukubola iibhondi ze-ester kunye neepeptides ezikufutshane ne-C-terminus yee-amino acids ze-hydrophobic, ii-amino acids eziqulethe i-sulfur, kunye neebhondi ze-aromatic amino acids, ezihlala zisetyenziselwa ukonakalisa iiproteni ukuvelisa iipeptides ezimfutshane. I-Proteinase K inophawu lwe-triad ye-catalytic eqhelekileyo i-Asp39-His69-Ser224 ekhethekileyo kwi-serine proteases kwaye ineendawo ezimbini zokubopha ze-Ca2+ ezijikeleze iziko elisebenzayo ukwandisa uzinzo lwayo kunye nokugcina umsebenzi ophezulu we-enzymatic phantsi koluhlu olubanzi lweemeko.
1. Isixhobo sokuchonga izifo zemfuza.
2. Izixhobo zokukhupha i-RNA kunye ne-DNA.
3. Khupha izinto ezingezizo iiproteni kwizicubu uze wonakalise ukungcola kweeproteni (njengokulungiselela ii-DNA vaccines kunye ne-heparin).
4. Ukulungiswa kweChromosomal DNA yePulse Electrophoresis.
5. Ukucinywa kweNtshona.
6. Uphuhliso kunye nokuveliswa kwee-enzymes ze-glycated albumin reagents kwicandelo lokuxilongwa kwe-in vitro.
1. 30mg/ibhotile 1g/ibhotile okanye 100g/ibhotile
2. 1kgs/ibhegi yefoyile
I-Proteinase K yi-protease ye-serine ezinzileyo eneenkcukacha ezibanzi ze-substrate. Yonakalisa iiproteni ezininzi kwimeko yayo nokuba kukho izicoci. Ayisebenziswa zii-ion zesinyithi, ii-agents ze-chelating (umzekelo, i-EDTA), ii-reagents ze-sulfhydryl, okanye zii-tripsin okanye ii-chymotrypsin inhibitors. Izinzile kuluhlu olubanzi lwe-pH (4–12.5), kunye nomsebenzi olungileyo kwi-pH 6.5–9.5. Umsebenzi unokukhuthazwa ngokongezwa kwee-agents ezinciphisa ubume (SDS kunye ne-Urea). Ukuncipha ngokukhawuleza kwe-enzyme kwenzeka kubushushu obungaphezulu kwama-70°C. Ukuguqulwa kwe-enzyme kwenzeka ngakumbi kwi-alkaline pH. Nangona kunjalo, i-Proteinase K ayisebenzi ngokupheleleyo yi-autolysis. Ezinye iziqwenga ze-enzyme ziyaqhubeka nokugcina umsebenzi wazo opheleleyo we-proteolytic, nangona emva kokuguqulwa okukhulu kwe-autolysis. I-Proteinase K isetyenziswa rhoqo kwizicelo ze-molecular biology ukugaya iiproteni ezingafunekiyo, ezifana nee-nucleases kumalungiselelo e-DNA okanye e-RNA avela kwii-microorganisms, iiseli ezikhuliswe, kunye nezityalo. Le enzyme idla ngokusetyenziswa kwi-50–200 ug/ml kumalungiselelo e-nucleic acid kwi-pH 7.5–8.0 kunye ne-37~55oC. Amaxesha okufukama ahluka ukusuka kwimizuzu engama-30 ukuya kwiiyure ezili-18.
I-serine protease ezinzileyo nesabela kakhulu; isetyenziselwa ukwahlulwa kweproteni kunye ne-nucleic acid. I-Proteinase K isetyenziswa ekucoceni i-RNA kunye ne-DNA kwizicubu okanye kwimigca yeseli.
Sizimisele ukubonelela ngexabiso eliphantsi, iimveliso kunye nezisombululo ezikumgangatho ophezulu, kunye nokuhanjiswa ngokukhawuleza kweAlkaline ProteinaseK kwifektri ngexabiso eliphantsi.I-CAS39450-01-6Besisoloko sifuna ukusebenzisana nabathengi kwihlabathi liphela. Sikholelwa ukuba siza kukwanelisa. Sikwabamkela ngobubele abathengi ukuba baye kumbutho wethu baze bathenge iimveliso zethu.
ixabiso eliphantsi lomzi-mvelisoI-China Alkaline ProteinaseK kunye ne-39450-01-6Ngenxa yomgangatho olungileyo kunye namaxabiso afanelekileyo, iimpahla zethu zithunyelwe kumazwe angaphezu kwe-10 nakwiindawo. Silangazelela ukusebenzisana nabo bonke abathengi basekhaya nakwamanye amazwe. Ngaphezu koko, ukwaneliseka kwabathengi yinto esiyifunayo ngonaphakade.
