I-Proteinase K CAS 39450-01-6 ethengiswa kakhulu kwifektri edumileyo kwihlabathi liphela enezinga eliphezulu lokuvezwa kunye nomhla wamva nje wokuveliswa kwayo
Ilungu ngalinye elivela kwiqela lethu elikhulu leengeniso zokusebenza lixabisa iimfuno zabathengi kunye nonxibelelwano lombutho kwiFactory ethengiswa kakhulu kwihlabathi liphela.I-Proteinase K CAS 39450-01-6Ngesantya Esiphezulu Sokuvezwa kunye noMhla Wamva Wokuveliswa, Okwangoku, sijonge phambili kwintsebenziswano enkulu nabathengi bamanye amazwe ngokusekelwe kwiingenelo ezifanayo. Qiniseka ukuba awuziva ukhululekile ukuqhagamshelana nathi ukuze ufumane ulwazi oluthe kratya.
Ilungu ngalinye elivela kwiqela lethu elikhulu leengeniso zokusebenza lixabisa iimfuno zabathengi kunye nonxibelelwano lombutho ukuzeITshayina 39450-01-6Siyakwamkela ngokufudumeleyo inkxaso-mali yakho kwaye siza kunceda abathengi bethu ekhaya nakwamanye amazwe ngeempahla ezikumgangatho ophezulu kunye nenkonzo egqwesileyo ejolise kwindlela yophuhliso oluqhubekayo njengesiqhelo. Sikholelwa ukuba uza kuxhamla kubungcali bethu kungekudala.
| Ulwelo lweNombolo 1 | |
| Umsebenzi othile weproteni>800U/mL, uxinzelelo yi-20mg/mL, akukho ntsalela yeNickase | |
| umgubo wenombolo 2 | |
| Inkangeleko | Umgubo omhlophe ocoliweyo |
| Ubumsulwa be-electrophoretic | ≥95% |
| Umsebenzi we-enzyme | ≥30U/mg |
| Intsalela ye-asidi ye-nucleic | Ayibonakali |
| Intsalela yeDeoxyribonuclease | Ayibonakali |
| Intsalela yeRibonuclease | Ayibonakali |
| Inombolo yenqwelo | G1205-10ML |
| Indawo yokugcina izinto | Xa igcinwe kwi--20 °C, imveliso igcina umsebenzi wayo ubuncinane unyaka omnye, ithuthwa ngeengxowa zomkhenkce. |
Iproteni Kyiprotease ye-serine ekwintsapho ye-subtilisin enomsebenzi we-enzyme osebenzayo kunye nokucaciswa kwe-substrate ebanzi, ekhetha ukubola iibhondi ze-ester kunye neepeptides ezikufutshane ne-C-terminus yee-amino acids ze-hydrophobic, ii-amino acids eziqulethe i-sulfur, kunye neebhondi ze-aromatic amino acids, ezihlala zisetyenziselwa ukonakalisa iiproteni ukuvelisa ii-peptides ezimfutshane.Iproteni Kinophawu lwe-catalytic triad eqhelekileyo i-Asp39-His69-Ser224 ekhethekileyo kwi-serine proteases kwaye ineendawo ezimbini zokubopha ze-Ca2+ ezijikeleze iziko elisebenzayo ukwandisa uzinzo lwayo kunye nokugcina umsebenzi ophezulu we-enzyme phantsi kweemeko ezahlukeneyo.
1. Isixhobo sokuchonga izifo zemfuza.
2. Izixhobo zokukhupha i-RNA kunye ne-DNA.
3. Khupha izinto ezingezizo iiproteni kwizicubu uze wonakalise ukungcola kweeproteni (njengokulungiselela ii-DNA vaccines kunye ne-heparin).
4. Ukulungiswa kweChromosomal DNA yePulse Electrophoresis.
5. Ukucinywa kweNtshona.
6. Uphuhliso kunye nokuveliswa kwee-enzymes ze-glycated albumin reagents kwicandelo lokuxilongwa kwe-in vitro.
1. 30mg/ibhotile 1g/ibhotile okanye 100g/ibhotile
2. 1kgs/ibhegi yefoyile
I-Proteinase K yi-protease ye-serine ezinzileyo eneenkcukacha ezibanzi ze-substrate. Yonakalisa iiproteni ezininzi kwimeko yayo nokuba kukho izicoci. Ayisebenziswa zii-ion zesinyithi, ii-agents ze-chelating (umzekelo, i-EDTA), ii-reagents ze-sulfhydryl, okanye zii-tripsin okanye ii-chymotrypsin inhibitors. Izinzile kuluhlu olubanzi lwe-pH (4–12.5), kunye nomsebenzi olungileyo kwi-pH 6.5–9.5. Umsebenzi unokukhuthazwa ngokongezwa kwee-agents ezinciphisa ubume (SDS kunye ne-Urea). Ukuncipha ngokukhawuleza kwe-enzyme kwenzeka kubushushu obungaphezulu kwama-70°C. Ukuguqulwa kwe-enzyme kwenzeka ngakumbi kwi-alkaline pH. Nangona kunjalo, i-Proteinase K ayisebenzi ngokupheleleyo yi-autolysis. Ezinye iziqwenga ze-enzyme ziyaqhubeka nokugcina umsebenzi wazo opheleleyo we-proteolytic, nangona emva kokuguqulwa okukhulu kwe-autolysis. I-Proteinase K isetyenziswa rhoqo kwizicelo ze-molecular biology ukugaya iiproteni ezingafunekiyo, ezifana nee-nucleases kumalungiselelo e-DNA okanye e-RNA avela kwii-microorganisms, iiseli ezikhuliswe, kunye nezityalo. Le enzyme idla ngokusetyenziswa kwi-50–200 ug/ml kumalungiselelo e-nucleic acid kwi-pH 7.5–8.0 kunye ne-37~55oC. Amaxesha okufukama ahluka ukusuka kwimizuzu engama-30 ukuya kwiiyure ezili-18.
I-serine protease ezinzileyo nesabela kakhulu; isetyenziselwa ukwahlulwa kweproteni kunye ne-nucleic acid. I-Proteinase K isetyenziswa ekucoceni i-RNA kunye ne-DNA kwizicubu okanye kwimigca yeseli.
Ilungu ngalinye elivela kwiqela lethu elikhulu leengeniso zokusebenza lixabisa iimfuno zabathengi kunye nonxibelelwano lombutho kwi-Factory ethengiswa kakhulu kwi-Globally Popular Proteinase K CAS39450-01-6Ngesantya Esiphezulu Sokuvezwa kunye noMhla Wamva Wokuveliswa, Okwangoku, sijonge phambili kwintsebenziswano enkulu nabathengi bamanye amazwe ngokusekelwe kwiingenelo ezifanayo. Qiniseka ukuba awuziva ukhululekile ukuqhagamshelana nathi ukuze ufumane ulwazi oluthe kratya.
Ivenkile ethengiswa kakhulu efektriITshayina 39450-01-6Siyakwamkela ngokufudumeleyo inkxaso-mali yakho kwaye siza kunceda abathengi bethu ekhaya nakwamanye amazwe ngeempahla ezisemgangathweni ophezulu kunye nenkonzo egqwesileyo ejolise kwindlela yophuhliso oluqhubekayo njengesiqhelo. Sikholelwa ukuba uza kuxhamla kubungcali bethu kungekudala.
